Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma

Marco Gaspari*, Mark Ming Cheng Cheng, Rosa Terracciano, Xuewu Liu, A. Jasper Nijdam, Lisa Vaccari, Enzo Di Fabrizio, Emanuel F. Petricoin, Lance A. Liotta, Giovanni Cuda, Salvatore Venuta, Mauro Ferrari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

Original languageEnglish (US)
Pages (from-to)1261-1266
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number5
DOIs
StatePublished - May 1 2006

Keywords

  • Human plasma
  • MALDI-TOF
  • Mass spectrometry
  • Nanoporous silica
  • Nanoporous silicon
  • Peptide profiling

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma'. Together they form a unique fingerprint.

Cite this