Nano-patterned SERS substrate: Application for protein analysis vs. temperature

Gobind Das*, Federico Mecarini, Francesco Gentile, Francesco De Angelis, HG G. Mohan Kumar, Patrizio Candeloro, Carlo Liberale, Giovanni Cuda, Enzo Di Fabrizio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

198 Scopus citations

Abstract

We have illustrated the fabrication of nano-structures as a surface enhanced Raman scattering (SERS) substrate using electro-plating and electron-beam lithography techniques to obtain an array of gold nanograin-aggregate structures of diameter ranging between 80 and 100 nm with interstitial gap of 10-30 nm. The nanostructure based SERS substrate permits us to have better control and reproducibility on generation of plasmon polaritons. The calculation shows the possible detection of myoglobin concentration down to attomole. This SERS substrate is used to investigate the structural changes of different proteins; lysozyme, ribonuclease-B, bovin serum albumin and myoglobin in the temperature range between -65 and 90 °C. The in-depth analysis even for small conformational changes is performed using 2D Raman correlation analysis and difference Raman analysis in order to gain straightforward understanding of proteins undergoing thermodynamical perturbation.

Original languageEnglish (US)
Pages (from-to)1693-1699
Number of pages7
JournalBiosensors and Bioelectronics
Volume24
Issue number6
DOIs
StatePublished - Feb 15 2009

Keywords

  • Difference and 2D-correlation Raman spectroscopy
  • Proteins
  • SERS
  • Temperature variation

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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