Multiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chip

Jinbo Wu, Mengying Zhang, Xiaolin Li, Weijia Wen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Microdroplets offer unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based microfluidics is the difficulty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a microfluidic chip to perform droplet screenings and aggressive reaction with minimal (nanoliter-scale) reagent consumption. The droplet composition, distance, volume (nanoliter to subnanoliter scale), number, and sequence could be precisely and digitally programmed through the improved sampling technique, while sample evaporation and cross-contamination are effectively eliminated. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. © 2012 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)9689-9693
Number of pages5
JournalAnalytical Chemistry
Volume84
Issue number22
DOIs
StatePublished - Nov 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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