Multimer resolution systems of ColE1 and ColK: localisation of the crossover site

David Summers*, Sami Yaish, John Archer, David Sherratt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We have identified and characterised a stability function encoded by the high copy plasmid ColK. The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state. In vivo recombination between cer and ckr (which share more than 90% homology at the DNA sequence level) produced a functional hybrid. Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry.

Original languageEnglish (US)
Pages (from-to)334-338
Number of pages5
JournalMGG Molecular & General Genetics
Volume201
Issue number2
DOIs
StatePublished - Nov 1985
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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