Multifunctional nanobeads based on quantum dots and magnetic nanoparticles: Synthesis and cancer cell targeting and sorting

Riccardo Di Corato, Nadja C. Bigall, Andrea Ragusa, Dirk Dorfs, Alessandro Genovese, Roberto Marotta, Liberato Manna, Teresa Pellegrino*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    153 Scopus citations

    Abstract

    Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).

    Original languageEnglish (US)
    Pages (from-to)1109-1121
    Number of pages13
    JournalACS Nano
    Volume5
    Issue number2
    DOIs
    StatePublished - Feb 22 2011

    Keywords

    • cell sorting
    • magnetic clusters
    • magnetic nanoparticles
    • magnetic-fluorescent nanostructures
    • quantumdots
    • tumor labeling

    ASJC Scopus subject areas

    • Materials Science(all)
    • Engineering(all)
    • Physics and Astronomy(all)

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