Multi-beam single-molecule defocused fluorescence imaging reveals local anisotropic nature of polymer thin films

Satoshi Habuchi*, Tatsuya Oba, Martin Vacha

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

We developed a method to determine full three-dimensional orientation distribution of individual molecules based on wide-field defocused fluorescence imaging. Excitation efficiencies of out-of-plane oriented molecules were improved dramatically by illuminating molecules with multiple laser beams. Our high throughput approach allowed us to obtain unbiased statistical distributions of orientations of doped molecules in spin-coated polymer thin films. We found thickness- and glass transition temperature-dependent distributions of the molecular orientations which reflect local chain orientations and relaxation in the polymer thin films.

Original languageEnglish (US)
Pages (from-to)7001-7007
Number of pages7
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number15
DOIs
StatePublished - Apr 21 2011

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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