Morphological characterization of virus-like particles in coral reef sponges

Cecília Pascelli, Patrick W. Laffy, Marija Kupresanin, Timothy Ravasi, Nicole S. Webster

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Marine sponges host complex microbial consortia that vary in their abundance, diversity and stability amongst host species. While our understanding of sponge-microbe interactions has dramatically increased over the past decade, little is known about how sponges and their microbial symbionts interact with viruses, the most abundant entities in the ocean. In this study, we employed three transmission electron microscopy (TEM) preparation methods to provide the first comprehensive morphological assessment of sponge-associated viruses. The combined approaches revealed 50 different morphologies of viral-like particles (VLPs) represented across the different sponge species. VLPs were visualized within sponge cells, within the sponge extracellular mesohyl matrix, on the sponge ectoderm and within sponge-associated microbes. Non-enveloped, non-tailed icosahedral VLPs were the most commonly observed morphotypes, although tailed bacteriophage, brick-shaped, geminate and filamentous VLPs were also detected. Visualization of sponge-associated viruses using TEM has confirmed that sponges harbor not only diverse communities of microorganisms but also diverse communities of viruses.
Original languageEnglish (US)
Pages (from-to)e5625
JournalPeerJ
Volume6
Issue number10
DOIs
StatePublished - Oct 17 2018

Fingerprint

Dive into the research topics of 'Morphological characterization of virus-like particles in coral reef sponges'. Together they form a unique fingerprint.

Cite this