Monolithic porous polymer stationary phases in polyimide chips for the fast high-performance liquid chromatography separation of proteins and peptides

Pavel A. Levkin, Sebastiaan Eeltink, Thomas R. Stratton, Reid Brennen, Karla Robotti, Hongfeng Yin, Kevin Killeen, Frantisek Svec, Jean Frechet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Poly(lauryl methacrylate-co-ethylene dimethacrylate) and poly(styrene-co-divinylbenzene) stationary phases in monolithic format have been prepared by thermally initiated free radical polymerization within polyimide chips featuring channels having a cross-section of 200 μm × 200 μm and a length of 6.8 cm. These chips were then used for the separation of a mixture of proteins including ribonuclease A, myoglobin, cytochrome c, and ovalbumin, as well as peptides. The separations were monitored by UV adsorption. Both the monolithic phases based on methacrylate and on styrene chemistries enabled the rapid baseline separation of most of the test mixtures. Best performance was achieved with the styrenic monolith leading to fast baseline separation of all four proteins in less than 2.5 min. The in situ monolith preparation process affords microfluidic devices exhibiting good batch-to-batch and injection-to-injection repeatability.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalJournal of Chromatography A
Volume1200
Issue number1
DOIs
StatePublished - Jul 18 2008

Keywords

  • Chip
  • HPLC
  • Microfluidics
  • Monoliths
  • Peptide separation
  • Protein separation
  • Proteomics
  • Stationary phase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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