Monolithic columns with a gradient of functionalities prepared via photoinitiated grafting for separations using capillary electrochromatography

Vicenzo Pucci, Maria Augusta Raggi, Frantisek Svec*, Jean Frechet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Stationary phases for capillary electrochromatography with a longitudinal gradient of functionalities have been prepared via photoinitiated grafting of polymer chains onto the pore surface of a porous polymer monolith. In order to achieve the desired retention and electroosmotic flow, the hydrophobic poly(butyl methacrylate-co-ethylene dimethacrylate) monolith with optimized porous properties was grafted with a layer of ionizable polymer, poly(2-acrylamido-2-methyl-1-propanesulfonic acid). A moving shutter and a neutral density filter were used to control the dose of UV light received at different locations along the monolith in order to create the longitudinal gradient of functionalities. Formation of the desired gradients was confirmed using electron probe microanalysis of different locations along the column. The preparation technique significantly affects performance in the CEC mode as demonstrated on the separations of a model mixture using columns both with homogeneous distribution of grafts and with a gradient of functionality. Columns grafted with the gradient of functionalities were found superior to those functionalized uniformly. A comparison of the performance of the gradient column with another containing evenly distributed functionalities showed the performance benefits of the "gradient" column.

Original languageEnglish (US)
Pages (from-to)779-788
Number of pages10
JournalJournal of Separation Science
Volume27
Issue number10-11
DOIs
StatePublished - Jul 1 2004

Keywords

  • Capillary electrochromatography
  • Column technology
  • Gradient of functionality
  • Monolith
  • Polymer
  • Stationary phase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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