Molecular mechanisms of RNA polymerase II transcription elongation elucidated by kinetic network models

Ilona Christy Unarta, Lizhe Zhu, Carmen Ka Man Tse, Peter Pak-Hang Cheung, Jin Yu, Xuhui Huang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Transcription elongation cycle (TEC) of RNA polymerase II (Pol II) is a process of adding a nucleoside triphosphate to the growing messenger RNA chain. Due to the long timescale events in Pol II TEC, an advanced computational technique, such as Markov State Model (MSM), is needed to provide atomistic mechanism and reaction rates. The combination of MSM and experimental results can be used to build a kinetic network model (KNM) of the whole TEC. This review provides a brief protocol to build MSM and KNM of the whole TEC, along with the latest findings of MSM and other computational studies of Pol II TEC. Lastly, we offer a perspective on potentially using a sequence dependent KNM to predict genome-wide transcription error.
Original languageEnglish (US)
Pages (from-to)54-62
Number of pages9
JournalCurrent Opinion in Structural Biology
Volume49
DOIs
StatePublished - Apr 2018
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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