Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis)

Thennarasu Sugumar, Pugalenthi Ganesan, Murugesan Harishankar, Gopal Dhinakar Raj

Research output: Contribution to journalArticlepeer-review

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.
Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalInternational Journal of Immunogenetics
Volume41
Issue number1
DOIs
StatePublished - Jun 25 2013

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

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