Modeling and simulation of luminescence detection platforms

Khaled Nabil Salama*, Helmy Eltoukhy, Arjang Hassibi, Abbas El Gamal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Motivated by the design of an integrated CMOS-based detection platform, a simulation model for CCD and CMOS imager-based luminescence detection systems is developed. The model comprises four parts. The first portion models the process of photon flux generation from luminescence probes using ATP-based and luciferase label-based assay kinetics. An optics simulator is then used to compute the incident photon flux on the imaging plane for a given photon flux and system geometry. Subsequently, the output image is computed using a detailed imaging sensor model that accounts for photodetector spectral response, dark current, conversion gain, and various noise sources. Finally, signal processing algorithms are applied to the image to enhance detection reliability and hence increase the overall system throughput. To validate the model, simulation results are compared to experimental results obtained from a CCD-based system that was built to emulate the integrated CMOS-based platform.

Original languageEnglish (US)
Pages (from-to)1377-1386
Number of pages10
JournalBiosensors and Bioelectronics
Volume19
Issue number11
DOIs
StatePublished - Jun 15 2004

Keywords

  • CMOS sensor
  • Luminescence probes
  • Modeling
  • Post-processing

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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