Modeling and simulation of integrated luminescence detection platforms

Khaled Nabil Salama*, Helmy Eltoukhy, Arjang Hassibi, Abbas El Gamal

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

5 Scopus citations

Abstract

We developed a simulation model of an integrated CMOS-based imaging platform for use with bioluminescent DNA microarrays. We formulate the complete kinetic model of ATP based assays and luciferase label-based assays. The model first calculates the number of photons generated per unit time, i.e., photon flux, based upon the kinetics of the light generation process of luminescence probes. The photon flux coupled with the system geometry is then used to calculate the number of photons incident on the photodetector plane. Subsequently the characteristics of the imaging array including the photodetector spectral response, its dark current density, and the sensor conversion gain are incorporated. The model also takes into account different noise sources including shot noise, reset noise, readout noise and fixed pattern noise. Finally, signal processing algorithms are applied to the image to enhance detection reliability and hence increase the overall system throughput. We will present simulations and preliminary experimental results.

Original languageEnglish (US)
Pages (from-to)106-116
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4966
DOIs
StatePublished - Dec 5 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis - San Jose, CA, United States
Duration: Jan 26 2003Jan 28 2003

Keywords

  • CMOS sensor
  • Luminescence probes
  • Modeling
  • Post-processing

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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