MMK2, a novel alfalfa MAP kinase, specifically complements the yeast MPK1 function

Claudia Jonak, Stefan Kiegerl, Heribert Hirt*, Clive Lloyd, Jordi Chan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Mitogen-activated protein (MAP) kinases are serine/threonine protein kinases that are activated in response to a variety of stimuli. Here we report the isolation of an alfalfa cDNA encoding a functional MAP kinase, termed MMK2. The predicted amino acid sequence of MMK2 shares 65% identity with a previously identified alfalfa MAP kinase, termed MMK1. Both alfalfa cDNA clones encode functional kinases when expressed in bacteria, undergoing autophosphorylation and activation to phosphorylate myelin basic protein in vitro. However, only MMK2 was able to phosphorylate a 39 kDa protein from the detergent-resistant cytoskeleton of carrot cells. The distinctiveness of MMK2 was further shown by complementation analysis of three different MAP kinase-dependent yeast pathways; this revealed a highly specific replacement of the yeast MPK1 (SLT2) kinase by MMK2, which was found to be dependent on activation by the upstream regulators of the pathway. These results establish the existence of MAP kinases with different characteristics in higher plants, suggesting the possibility that they could mediate different cellular responses.

Original languageEnglish (US)
Pages (from-to)686-694
Number of pages9
JournalMgg Molecular & General Genetics
Volume248
Issue number6
DOIs
StatePublished - Oct 1 1995

Keywords

  • Alfalfa
  • MAP kinase
  • MPK1
  • Serine/threonine protein kinase
  • Signal transduction

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'MMK2, a novel alfalfa MAP kinase, specifically complements the yeast MPK1 function'. Together they form a unique fingerprint.

Cite this