mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells

Daming Zhang, Guang Yang, Xin Chen, Chunmei Li, Lu Wang, Yaohua Liu, Dayong Han, Huailei Liu, Xu Hou, Weiguang Zhang, Chenguang Li, Zhanqiang Han, Xin Gao, Shiguang Zhao

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
Original languageEnglish (US)
Pages (from-to)637-644
Number of pages8
JournalJournal of Molecular Neuroscience
Volume53
Issue number4
DOIs
StatePublished - Jan 28 2014

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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