Microbial community profiling using terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE)

José F. Siqueira, Mitsuo Sakamoto, Alexandre S. Rosado

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

In their natural environments, microorganisms usually live in organized communities. Profiling analysis of microbial communities has recently assumed special relevance as it allows a thorough understanding of the diversity of the microbiota, its behavior over time, and the establishment of patterns associated with health and disease. The application of molecular biology approaches holds the advantage of including culturedifficult and as-yet- uncultivated phylotypes in the profiles, providing a more comprehensive picture of the microbial community. This chapter focuses on two particular techniques, namely, terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE), both of which have been widely used in environmental studies and have been successfully used by the authors in the study of the oral microbial communities associated with conditions of health and disease.
Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.humana@humanapr.com
Pages139-152
Number of pages14
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

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