As a first step towards a disposable diagnostic microchip for determination of urinary human serum albumin (HSA), we report the use of a thin-film organic light emitting diode (OLED) as an excitation source for microscale fluorescence detection. The OLED has a peak emission at 540 nm, is simple to fabricate and operates at drive voltages below 10 V. In a PDMS microchip, HSA was reacted with Albumin Blue 580, generating a strong emission at 620 nm when excited with the OLED. HSA concentrations down to 10 mg/L could be detected, enabling the determination of microalbuminuria (MAU), an increased urinary albumin excretion indicative of renal disease. Copyright © 2005 by the Transducer Research Foundation, Inc.
|Original language||English (US)|
|Title of host publication||Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Publisher||Transducer Research Foundationinfo@transducer-research-foundation.org|
|State||Published - Jan 1 2005|