Marker-free phase nanoscopy

Yann Cotte*, Fatih Toy, Pascal Jourdain, Nicolas Pavillon, Daniel Boss, Pierre Magistretti, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

400 Scopus citations

Abstract

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured using interferometric detection, allowing diffraction-limited phase measurements. Here, we show that non-invasive optical nanoscopy can achieve a lateral resolution of 90 nm by using a quasi-2π-holographic detection scheme and complex deconvolution. We record holograms from different illumination directions on the sample plane and observe subwavelength tomographic variations of the specimen. Nanoscale apertures serve to calibrate the tomographic reconstruction and to characterize the imaging system by means of the coherent transfer function. This gives rise to realistic inverse filtering and guarantees true complex field reconstruction. The observations are shown for nanoscopic porous cell frustule (diatoms), for the direct study of bacteria (Escherichia coli), and for a time-lapse approach to explore the dynamics of living dendritic spines (neurones).

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalNature Photonics
Volume7
Issue number2
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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