Marine Microorganisms as Source of Stereoselective Esterases and Ketoreductases: Kinetic Resolution of a Prostaglandin Intermediate

Valerio De Vitis, Benedetta Guidi, Martina Letizia Contente, Tiziana Mariarita Granato, Paola Conti, Francesco Molinari, Elena Crotti, Francesca Mapelli, Sara S. Borin, Daniele Daffonchio, Diego Romano

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
Original languageEnglish (US)
Pages (from-to)144-152
Number of pages9
JournalMarine Biotechnology
Volume17
Issue number2
DOIs
StatePublished - Sep 30 2014

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

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