TY - JOUR
T1 - Mapping of novel salt tolerance QTL in an Excalibur × Kukri doubled haploid wheat population
AU - Asif, Muhammad A.
AU - Schilling, Rhiannon K.
AU - Tilbrook, Joanne
AU - Brien, Chris
AU - Dowling, Kate
AU - Rabie, Huwaida
AU - Short, Laura
AU - Trittermann, Christine
AU - Garcia, Alexandre
AU - Barrett-Lennard, Edward G.
AU - Berger, Bettina
AU - Mather, Diane E.
AU - Gilliham, Matthew
AU - Fleury, Delphine
AU - Tester, Mark A.
AU - Roy, Stuart J.
AU - Pearson, Allison S.
N1 - KAUST Repository Item: Exported on 2021-02-19
Acknowledgements: This project was funded by the Grains Research and Development Corporation (GRDC): Project UA00118 to MT, SJR; UA00145 to SJR, BB, MG and EGB-L; and UA00159 to SJR and MG. We also acknowledge the South Australian growers and Cunderdin Agricultural College, Western Australia who provided land for field trials, South Australian Research and Development Institute (SARDI) new varieties agronomy team, Kalyx Australia and Mr. David Farleigh (DPIRD), Mr. Rob Jeffrey (UWA), Dr. Louis Mayer (UWA), Dr. Victoria Marchesini (UWA), Dr. Christa Niemietz (UofA), Dr. Jiaen Qiu (UofA) and Mr. Nigel Schilling (UofA) for field trial assistance, and The Plant Accelerator® staff who assisted with the glasshouse experiments. The Plant Accelerator®, Australian Plant Phenomics Facility, is funded under the National Collaborative Research Infrastructure Strategy (NCRIS). The bread wheat genome assembly was accessed through the International Wheat Genome Sequencing Consortium at https://wheat-urgi.versailles.inra.fr/Seq-Repository/Assemblies. MAA thanks the University of Adelaide for Adelaide Scholarships International (ASI) and the Australian Centre for Plant Functional Genomics for his PhD stipend, also acknowledged the financial support from the Australian Society of Plant Scientists (ASPS), the Crop Science Society of South Australia Incorporated and the Plant Nutrition Trust to attend conferences. MT thanks King Abdullah University of Science and Technology (KAUST) for financial support.
PY - 2018/7/30
Y1 - 2018/7/30
N2 - Key messageNovel QTL for salinity tolerance traits have been detected using non-destructive and destructive phenotyping in bread wheat and were shown to be linked to improvements in yield in saline fields.AbstractSoil salinity is a major limitation to cereal production. Breeding new salt-tolerant cultivars has the potential to improve cereal crop yields. In this study, a doubled haploid bread wheat mapping population, derived from the bi-parental cross of Excalibur × Kukri, was grown in a glasshouse under control and salinity treatments and evaluated using high-throughput non-destructive imaging technology. Quantitative trait locus (QTL) analysis of this population detected multiple QTL under salt and control treatments. Of these, six QTL were detected in the salt treatment including one for maintenance of shoot growth under salinity (QG(1–5).asl-7A), one for leaf Na+ exclusion (QNa.asl-7A) and four for leaf K+ accumulation (QK.asl-2B.1, QK.asl-2B.2, QK.asl-5A and QK:Na.asl-6A). The beneficial allele for QG(1–5).asl-7A (the maintenance of shoot growth under salinity) was present in six out of 44 mainly Australian bread and durum wheat cultivars. The effect of each QTL allele on grain yield was tested in a range of salinity concentrations at three field sites across 2 years. In six out of nine field trials with different levels of salinity stress, lines with alleles for Na+ exclusion and/or K+ maintenance at three QTL (QNa.asl-7A, QK.asl-2B.2 and QK:Na.asl-6A) excluded more Na+ or accumulated more K+ compared to lines without these alleles. Importantly, the QK.asl-2B.2 allele for higher K+ accumulation was found to be associated with higher grain yield at all field sites. Several alleles at other QTL were associated with higher grain yields at selected field sites.
AB - Key messageNovel QTL for salinity tolerance traits have been detected using non-destructive and destructive phenotyping in bread wheat and were shown to be linked to improvements in yield in saline fields.AbstractSoil salinity is a major limitation to cereal production. Breeding new salt-tolerant cultivars has the potential to improve cereal crop yields. In this study, a doubled haploid bread wheat mapping population, derived from the bi-parental cross of Excalibur × Kukri, was grown in a glasshouse under control and salinity treatments and evaluated using high-throughput non-destructive imaging technology. Quantitative trait locus (QTL) analysis of this population detected multiple QTL under salt and control treatments. Of these, six QTL were detected in the salt treatment including one for maintenance of shoot growth under salinity (QG(1–5).asl-7A), one for leaf Na+ exclusion (QNa.asl-7A) and four for leaf K+ accumulation (QK.asl-2B.1, QK.asl-2B.2, QK.asl-5A and QK:Na.asl-6A). The beneficial allele for QG(1–5).asl-7A (the maintenance of shoot growth under salinity) was present in six out of 44 mainly Australian bread and durum wheat cultivars. The effect of each QTL allele on grain yield was tested in a range of salinity concentrations at three field sites across 2 years. In six out of nine field trials with different levels of salinity stress, lines with alleles for Na+ exclusion and/or K+ maintenance at three QTL (QNa.asl-7A, QK.asl-2B.2 and QK:Na.asl-6A) excluded more Na+ or accumulated more K+ compared to lines without these alleles. Importantly, the QK.asl-2B.2 allele for higher K+ accumulation was found to be associated with higher grain yield at all field sites. Several alleles at other QTL were associated with higher grain yields at selected field sites.
UR - http://hdl.handle.net/10754/628782
UR - http://link.springer.com/article/10.1007/s00122-018-3146-y
UR - http://www.scopus.com/inward/record.url?scp=85053412281&partnerID=8YFLogxK
U2 - 10.1007/s00122-018-3146-y
DO - 10.1007/s00122-018-3146-y
M3 - Article
C2 - 30062653
AN - SCOPUS:85053412281
VL - 131
SP - 2179
EP - 2196
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
SN - 0040-5752
IS - 10
ER -