Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

Silvia Nappini, Tamer Al Kayal, Debora Berti, Bengt Nordèn, Piero Baglioni

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)713-718
Number of pages6
JournalThe Journal of Physical Chemistry Letters
Volume2
Issue number7
DOIs
StatePublished - Mar 10 2011
Externally publishedYes

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