Living specimen tomography by digital holographic microscopy: Morphometry of testate amoeba

Florian Charrière*, Nicolas Pavillon, Tristan Colomb, Christian Depeursinge, Thierry J. Heger, Edward A.D. Mitchell, Pierre Marquet, Benjamin Rappaz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

224 Scopus citations

Abstract

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of n, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20x magnification allows a resolution better than 3 μm in all three dimensions, with accuracy better than 0.01 for the refractive index measurements. This technique is for the first time to our knowledge applied to living specimen (testate amoeba, Protista). Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%.

Original languageEnglish (US)
Pages (from-to)7005-7013
Number of pages9
JournalOptics Express
Volume14
Issue number16
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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