Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains

Michihiro Ogasawara, Tadashi Imanishi, Kazuo Moriwaki, Silvana Gaudieri, Hiroshi Tsuda, Hiroshi Hashimoto, Toshihiko Shiroishi, Takashi Gojobori*, Tsuyoshi Koide

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

CAG repeats coding for poly-glutamines have been studied by many groups as repeat length variations contributes to differences in protein function and disease outcome. In this study, we systematically searched public databases for genes carrying CAG repeats. For the genes obtained, we experimentally analyzed variations of length and the purity of the repeats in 62 loci among 16 inbred mouse strains, including wild-derived and laboratory strains. We found that length was conserved in 50% of the loci, especially among wild-derived strains. Of 496 polymorphic repeat alleles, 78% were uninterrupted and 22% were interrupted with non-CAG codons. Interruptions tended to occur in longer repeats and all repeats of greater length than 23 were interrupted. Although interruptions can act as suppressors for the expansion of CAG repeats, we found that the occurrence of the interruptions depended on the length of the CAG repeats. Furthermore, most poly-glutamines examined in this study existed in human orthologous genes, reflecting the functional significance of poly-glutamines in proteins.

Original languageEnglish (US)
Pages (from-to)107-119
Number of pages13
JournalGene
Volume349
DOIs
StatePublished - Apr 11 2005

Keywords

  • Evolution
  • Functional constraint
  • Poly-glutamines
  • Wild mice

ASJC Scopus subject areas

  • Genetics

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