LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technology.

Patrick Driguez, Salim Bougouffa, Karen Carty, Alexander Putra, Kamel Jabbari, Muppala P. Reddy, Richard Willem Otto Soppe, Ming Sin Cheung, Yoshinori Fukasawa, Luca Ermini

Research output: Contribution to journalArticlepeer-review

Abstract

Currently, different sequencing platforms are used to generate plant genomes and no workflow has been properly developed to optimize time, cost, and assembly quality. We present LeafGo, a complete de novo plant genome workflow, that starts from tissue and produces genomes with modest laboratory and bioinformatic resources in approximately 7 days and using one long-read sequencing technology. LeafGo is optimized with ten different plant species, three of which are used to generate high-quality chromosome-level assemblies without any scaffolding technologies. Finally, we report the diploid genomes of Eucalyptus rudis and E. camaldulensis and the allotetraploid genome of Arachis hypogaea.
Original languageEnglish (US)
JournalGenome biology
Volume22
Issue number1
DOIs
StatePublished - Sep 4 2021

ASJC Scopus subject areas

  • Genetics

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