Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Etienne Shaffer*, Corinne Moratal, Pierre Magistretti, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

Original languageEnglish (US)
Pages (from-to)4102-4104
Number of pages3
JournalOptics Letters
Volume35
Issue number24
DOIs
StatePublished - Dec 15 2010
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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