Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Zhijuan Wang, Juan Zhang, Peng Chen, Xiaozhu Zhou, Yanli Yang, Shixin Wu, Li Niu, Yu Han, Lianhui Wang, Peng Chen, Freddy Boey, Qichun Zhang, Bo Gunnar Liedberg, Hua Zhang

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity. © 2011 Elsevier B.V.
Original languageEnglish (US)
Pages (from-to)3881-3886
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number9
DOIs
StatePublished - May 2011

ASJC Scopus subject areas

  • Biomedical Engineering
  • Electrochemistry
  • Biotechnology
  • Biophysics

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