Lab on a chip automates in vitro cell culturing

Gerardo Perozziello*, Jacob Møllenbach, Steen Laursen, Enzo Di Fabrizio, Krist Gernaey, Ulrich Krühne

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 μl/h. A common pH electrode is supplied by 14 μl sample, which is expanded with help of DI water.

Original languageEnglish (US)
Pages (from-to)655-658
Number of pages4
JournalMicroelectronic Engineering
Volume98
DOIs
StatePublished - Oct 1 2012

Keywords

  • Automated in vitro fertilization
  • Lab on a chip
  • Rapid prototyping
  • Single cell cultivation
  • T and pH control

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering

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