Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells

Christoph Gehring, H. R. Irving, R. McConchie, R. W. Parish*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Jasmonates (jasmonic acid or methyl jasmonate) promote stomatal closure in Paphiopedilum Supersuk (RHS, 1973) and P, tonsum (Rchb.f) Stein. Studies on guard cells loaded with pH dependent fluorescent dyes show that jasmonates cause intracellular alkalinization of up to 0.5 pH units within 5 to 15 min. Jasmonate-induced alkalinization always preceded stomatal closure and where alkalinization was not detected no closure occurred. Propionic acid inhibited jasmonate-induced stomatal closure, suggesting that jasmonate-induced intracellular alkalinization is involved in guard cell movements.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalAnnals of Botany
Volume80
Issue number4
DOIs
StatePublished - Jan 1 1997

Keywords

  • BCECF
  • Confocal microscopy
  • Cytosolic pH
  • Guard cells
  • Jasmonic acid
  • Methyl jasmonate
  • Paphiopedilum Supersuk (R. H. S.)
  • Snarf-1
  • Stomatal movements

ASJC Scopus subject areas

  • Plant Science

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