Isolation of UVC-Tolerant Bacteria from the Hyperarid Atacama Desert, Chile

Ivan Gláucio Paulino-Lima, Armando Azua-Bustos, Rafael Vicuña, Carlos González-Silva, Loreto Salas, Lia Teixeira, Alexandre Rosado, Alvaro Augusto da Costa Leitao, Claudia Lage

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Martian surface microbial inhabitants would be challenged by a constant and unimpeded flux of UV radiation, and the study of analog model terrestrial environments may be of help to understand how such life forms could survive under this stressful condition. One of these environments is the Atacama Desert (Chile), a well-known Mars analog due to its extreme dryness and intense solar UV radiation. Here, we report the microbial diversity at five locations across this desert and the isolation of UVC-tolerant microbial strains found in these sites. Denaturing gradient gel electrophoresis (DGGE) of 16S rDNA sequences obtained from these sites showed banding patterns that suggest distinct and complex microbial communities. Analysis of 16S rDNA sequences obtained from UV-tolerant strains isolated from these sites revealed species related to the Bacillus and Pseudomonas genera. Vegetative cells of one of these isolates, Bacillus S3. 300-2, showed the highest UV tolerance profile (LD10 = 318 J m2), tenfold higher than a wild-type strain of Escherichia coli. Thus, our results show that the Atacama Desert harbors a noteworthy microbial community that may be considered for future astrobiological-related research in terms of UV tolerance. © 2012 Springer Science+Business Media, LLC.
Original languageEnglish (US)
Pages (from-to)325-335
Number of pages11
JournalMicrobial Ecology
Volume65
Issue number2
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

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