Isolation of Elaeagnus-compatible Frankia from soils collected in Tunisia

Maher Gtari, Lorenzo Brusetti, Gharbi Skander, Diego Mora, Abdellatif Boudabous, Daniele Daffonchio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalFEMS Microbiology Letters
Volume234
Issue number2
DOIs
StatePublished - May 15 2004

Keywords

  • Elaeagnus
  • Frankia
  • Genetic diversity
  • Isolated strains
  • Soil
  • Tunisia

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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