Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Stefania De Vitis, Giuseppina Matarise, Francesca Pardeo, Rossella Catalano, Natalia Maria Malara, Valentina Trunzo, Rossana Tallerico, Francesco T. Gentile, Patrizio Candeloro, Maria Laura Coluccio, Alessandro S. Massaro, Giuseppe Viglietto, Ennio Carbone, Jörg Peter Kutter, Gerardo Perozziello, Enzo M. Di Fabrizio

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.
Original languageEnglish (US)
Pages (from-to)76-80
Number of pages5
JournalMicroelectronic Engineering
Volume124
DOIs
StatePublished - Jul 2014

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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