Isolation and characterization of plant protein complexes by mass spectrometry

Delphine Pflieger, Jean Bigeard, Heribert Hirt*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

18 Scopus citations

Abstract

The components that enable cells and organisms to fulfill a plethora of chemical and physical reactions, including their ability to metabolize, replicate, repair and communicate with their environment are mostly based on the functioning of highly complex cellular machines which are to a large extent composed of proteins. With the development of MS techniques compatible with the analysis of minute amounts of biological material, it has become more and more feasible to dissect the composition and modification of these protein machineries. Indeed, new purification methods of protein complexes followed by MS analysis together with the genomic sequencing of various organisms - and in particular of crop species - now provide unforeseen insight to understand biological processes at a molecular level. We here review the current state of the art of in vivo protein complex isolation and their MS-based analytical characterization, emphasizing on the tandem affinity purification approach.

Original languageEnglish (US)
Pages (from-to)1824-1833
Number of pages10
JournalProteomics
Volume11
Issue number9
DOIs
StatePublished - May 9 2011

Keywords

  • MS
  • Plant proteomics
  • Protein complexes
  • Tandem affinity purification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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