Isolation and characterization of phosphoprotein phosphatase 1 from alfalfa

Aniko Pay, Manfred Pirck, Laszlo Bögre, Heribert Hirt*, Erwin Heberle-Bors

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Protein phosphatases are central regulatory components of diverse processes in eukaryotes and are among the most highly conserved proteins known. In this paper, we report the cloning and sequencing of a type 1 protein phosphatase (pp1Ms) cDNA from alfalfa. Southern analysis indicates the presence of a gene family of PP1 proteins in alfalfa. The pp1Ms open reading frame is very similar to one of five predicted Arabidopsis type 1 protein phosphatases, indicating that different subtypes are individually conserved. Expression of the alfalfa pp1Ms in a temperature-sensitive Schizosaccharomyces pombe PP1 mutant, dis2-11, revealed no complementation, suggesting that PP1Ms is not involved in mitotic regulation. In different plant organs, different pp1Ms transcript levels were observed; in contrast, mRNA levels remained constant in all phases of the cell cycle and in logarithmically growing cells. However, when cells entered stationary phase pp1Ms transcript levels decreased considerably.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalMGG Molecular & General Genetics
Volume244
Issue number2
DOIs
StatePublished - Mar 1994
Externally publishedYes

Keywords

  • Cell Cycle Alfalfa
  • Phosphoprotein Phosphatase

ASJC Scopus subject areas

  • Genetics

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