Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

F. Alberto*, L. Correia, C. Billot, Carlos Duarte, E. Serrão

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

Original languageEnglish (US)
Pages (from-to)397-399
Number of pages3
JournalMolecular Ecology Notes
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2003

Keywords

  • Clonal plant
  • Cymodocea nodosa
  • Microsatellites
  • Seagrass

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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