Investigation over the life cycle of ST1-C the endosymbiont of Scaphoideus titanus

Massimo Pajoro, Massimo Marzorati, Ilaria Negri, Luciano Sacchi, Daniele Daffonchio, Alberto Alma*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Scaphoideus titanus Ball (Hemiptera Cicadellidae) is a phloem-feeder leafhopper vector of 'Candidatus Phytoplasma vitis', the phytoplasma responsible of "flavescence dorée" (FD), a grape yellows afflicting several wine production areas in Europe. Natural symbionts of the leafhopper for symbiotic control of FD transmission could be used. Previous work showed the presence of a CFB bacterium, 'Candidatus Cardinium sp.' (named ST1-C) in several body districts of S. titanus. Our work was aimed to investigate the life cycle of ST1-C endosymbiont inside and outside of insect body. We fed S. titanus individuals on artificial media and tested insects and diets after the treatment for the presence of the ST1-C endosymbiont by qualitative and quantitative PCR, showing the release of the bacterium during feeding. We also performed fluorescent in situ hybridization on the sugar solutions after insect feeding, detecting ST1-C cells that confirmed its release into the medium. We tested by ST1-C-specific PCR DNA extracted from small areas of grape leaves exposed to S. titanus, identifying the bacterium in the majority of pierced leaves. Our experiments suggest that the ST1-C endosymbiont can undergo a horizontal transmission and have a complex life cycle possibly involving more than one host.

Original languageEnglish (US)
Pages (from-to)217-218
Number of pages2
JournalBulletin of Insectology
Volume61
Issue number1
StatePublished - 2008
Externally publishedYes

Keywords

  • Cardinium
  • Flavescence dorée
  • Horizontal transmission
  • Scaphoideus titanus
  • Symbiotic control

ASJC Scopus subject areas

  • Insect Science

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