Insight into the genome of Aspergillus fumigatus: Analysis of a 922 kb region encompassing the nitrate assimilation gene cluster

Arnab Pain, John Woodward, Michael A. Quail, Michael J. Anderson, Richard Clark, Matthew Collins, Nigel Fosker, Audrey Fraser, David Harris, Natasha Larke, Lee Murphy, Sean Humphray, Susan O'Neil, Mihaela Pertea, Claire Price, Ester Rabbinowitsch, Marie Adele Rajandream, Steven Salzberg, David Saunders, Kathy SeegerSarah Sharp, Tim Warren, David W. Denning, Bart Barrell, Neil Hall*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be ∼30Mb in size, we have sequenced a 922kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.

Original languageEnglish (US)
Pages (from-to)443-453
Number of pages11
JournalFungal Genetics and Biology
Volume41
Issue number4
DOIs
StatePublished - Apr 1 2004

Keywords

  • Annotation
  • Aspergillus fumigatus
  • BAC-end sequencing
  • Comparative genomics
  • Gene-cluster
  • Genome
  • Synteny

ASJC Scopus subject areas

  • Microbiology
  • Genetics

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