Inhibitory effect of common microfluidic materials on PCR outcome

Rimantas Kodzius, Kang Xiao, Jinbo Wu, Xin Yi, Xiuqing Gong, Ian G. Foulds, Weijia Wen

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

In this study, we established a simple method for evaluating the PCR compatibility of various common materials employed when fabricating microfluidic chips, including silicon, several kinds of silicon oxide, glasses, plastics, wax, and adhesives. Two-temperature PCR was performed with these materials to determine their PCR-inhibitory effect. In most cases, adding bovine serum albumin effectively improved the reaction yield. We also studied the individual PCR components from the standpoint of adsorption. Most of the materials did not inhibit the DNA, although they noticeably interacted with the polymerase. We provide a simple method of performing PCR-compatibility testing of materials using inexpensive instrumentation that is common in molecular biology laboratories. Furthermore, our method is direct, being performed under actual PCR conditions with high temperature. Our results provide an overview of materials that are PCR-friendly for fabricating microfluidic devices. The PCR reaction, without any additives, performed best with pyrex glass, and it performed worst with PMMA or acrylic glue materials.
Original languageEnglish (US)
Pages (from-to)349-358
Number of pages10
JournalSensors and Actuators B: Chemical
Volume161
Issue number1
DOIs
StatePublished - Oct 28 2011

ASJC Scopus subject areas

  • Materials Chemistry
  • Instrumentation
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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