INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

Ronny Volz, SoonKap Kim, Jianing Mi, Anamika Rawat, Alaguraj Veluchamy, Kiruthiga Mariappan, Naganand Rayapuram, Jean-Michel Daviere, Patrick Achard, Ikram Blilou, Salim Al-Babili, Moussa Benhamed, Heribert Hirt

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of basal immune response and PAMP-triggered immunity. Furthermore, the idd4 mutant exhibits enhanced plant-growth indicating IDD4 as suppressor of growth and development. Transcriptome comparison of idd4 mutants and IDD4ox lines aligned to genome-wide IDD4 DNA-binding studies revealed major target genes related to defense and developmental-biological processes. IDD4 is a phospho-protein that interacts and becomes phosphorylated on two conserved sites by the MAP kinase MPK6. DNA-binding studies of IDD4 after flg22 treatment and with IDD4 phosphosite mutants show enhanced binding affinity to ID1 motif-containing promoters and its function as a transcriptional regulator. In contrast to the IDD4-phospho-dead mutant, the IDD4 phospho-mimicking mutant shows altered susceptibility to PstDC3000, salicylic acid levels and transcriptome reprogramming. In summary, we found that IDD4 regulates various hormonal pathways thereby coordinating growth and development with basal immunity.
Original languageEnglish (US)
Pages (from-to)e1007499
JournalPLOS Pathogens
Volume15
Issue number1
DOIs
StatePublished - Jan 24 2019

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Virology
  • Molecular Biology
  • Microbiology
  • Parasitology

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