In vivo measurement of glucose utilization in rats using a β-microprobe: Direct comparison with autoradiography

Philippe Millet*, Marcelle Moulin Sallanon, Jean Marie Petit, Yves Charnay, Philippe Vallet, Christian Morel, Raymond Cespuglio, Pierre Magistretti, Vicente Ibáñez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A new β-microprobe (βP) has been used to locally measure the time-concentration curve of a radiolabeled substance. The βP, analogous to positron emission tomography methodology, is useful for in vivo animal studies because it can acquire time-concentration curves with high temporal and spatial resolution. Using [ 18F]fluoro-2-deoxy-D-glucose and βP, we evaluated the reliability of the biologic parameters and we compared this method with the [ 14C]2-deoxy-D-glucose autoradiography. βP time-concentration curves in three regions of the brain were obtained from 24 rats. Four kinetic parameters (K 1-k 4) were estimated from 60-minute experimental periods using a three-compartment model. Best fits were obtained when the vascular fraction (F v) was estimated simultaneously with the four kinetic parameters (K 1-k 4). The mean estimated F v values were about 5.5% for the frontal cortex regions and 8.0% for the cerebellum. Correlation coefficients higher than 0.830 were observed between regional cerebral metabolic rates for glucose (rCMRglc) values obtained by βP and autoradiography. In addition, the βP-derived input function was similar to that obtained by manual sampling. Our findings show that reliable rCMRglc values can be obtained using βP.

Original languageEnglish (US)
Pages (from-to)1015-1024
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume24
Issue number9
DOIs
StatePublished - Sep 1 2004

Keywords

  • Autoradiography
  • Mathematical modeling
  • rCMRglc
  • β-microprobe

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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