In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata

M. M. Maryani, M. V. Morse, G. Bradley, H. R. Irving, D. M. Cahill, Christoph Gehring*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Plant natriuretic peptide immuno-analogues (irPNP) have previously been shown to affect a number of biological processes including stomatal guard cell movements, ion fluxes and osmoticum-dependent water transport. Tissue printing and immunofluorescent labelling techniques have been used here to study the tissue and cellular localization of irPNP in ivy (Hedera helix L.) and potato (Solanum tuberosum L.). Polyclonal antibodies active against human atrial natriuretic peptide (anti-hANP) and antibodies against irPNP from potato (anti-StPNP) were used for immunolabelling. Tissue prints revealed that immunoreactants are concentrated in vascular tissues of leaves, petioles and stems. Phloem-associated cells, xylem cells and parenchymatic xylem cells showed the strongest immunoreaction. Immunofluorescent microscopy with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG supported this finding and, furthermore, revealed strong labelling to stomatal guard cells and the adjacent apoplastic space as well. Biologically active immunoreactants were also detected in xylem exudates of a soft South African perennial forest sage (Plectranthus ciliatus E. Mey ex Benth.) thus strengthening the evidence for a systemic role of the protein. In summary, in situ cellular localization is consistent with physiological responses elicited by irPNPs reported previously and is indicative of a systemic role in plant homeostasis.

Original languageEnglish (US)
Pages (from-to)1553-1564
Number of pages12
JournalJournal of Experimental Botany
Volume54
Issue number387
DOIs
StatePublished - Jun 1 2003

Keywords

  • Conductive tissue
  • Expansins
  • Hedera helix L.
  • Homeostasis
  • Immuno-analogues
  • Plant natriuretic peptides
  • Plectranthus ciliatus E. Mey ex Benth.
  • Solanum tuberosum L.
  • Tissue printing

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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