In-line balanced detection stimulated Raman scattering microscopy

Francesco Crisafi, Vikas Kumar, Tullio Scopigno, Marco Marangoni, Giulio Cerullo, Dario Polli

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.
Original languageEnglish (US)
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Sep 6 2017
Externally publishedYes

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