Improved plant resistance to drought is promoted by the root-associated microbiome as a water stress-dependent trait

Eleonora Rolli, Ramona Marasco, Gianpiero Vigani, Besma Ettoumi, Francesca Mapelli, Maria Laura Deangelis, Claudio Gandolfi, Enrico Casati, Franco Previtali, Roberto Gerbino, Fabio Pierotti Cei, Sara Borin, Claudia Sorlini, Graziano Zocchi, Daniele Daffonchio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

Although drought is an increasing problem in agriculture, the contribution of the root-associated bacterial microbiome to plant adaptation to water stress is poorly studied. We investigated if the culturable bacterial microbiome associated with five grapevine rootstocks and the grapevine cultivar Barbera may enhance plant growth under drought stress. Eight isolates, over 510 strains, were tested in vivo for their capacity to support grapevine growth under water stress. The selected strains exhibited a vast array of plant growth promoting (PGP) traits, and confocal microscopy observation of gfp-labelled Acinetobacter and Pseudomonas isolates showed their ability to adhere and colonize both the Arabidopsis and grapevine rhizoplane. Tests on pepper plants fertilized with the selected strains, under both optimal irrigation and drought conditions, showed that PGP activity was a stress-dependent and not a per se feature of the strains. The isolates were capable of increasing shoot and leaf biomass, shoot length, and photosynthetic activity of drought-challenged grapevines, with an enhanced effect in drought-sensitive rootstock. Three isolates were further assayed for PGP capacity under outdoor conditions, exhibiting the ability to increase grapevine root biomass. Overall, the results indicate that PGP bacteria contribute to improve plant adaptation to drought through a water stress-induced promotion ability.

Original languageEnglish (US)
Pages (from-to)316-331
Number of pages16
JournalEnvironmental Microbiology
Volume17
Issue number2
DOIs
StatePublished - Feb 1 2015

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint Dive into the research topics of 'Improved plant resistance to drought is promoted by the root-associated microbiome as a water stress-dependent trait'. Together they form a unique fingerprint.

Cite this