Identification of constitutively active AtMPK6 mutants using a functional screen in saccharomyces cerevisiae

Elodie Hudik, Souha Berriri, Heribert Hirt, Jean Colcombet

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast.

Original languageEnglish (US)
Pages (from-to)67-77
Number of pages11
JournalMethods in Molecular Biology
Volume1171
DOIs
StatePublished - Jan 1 2014

Keywords

  • Constitutive activity
  • Functional genetic screen
  • In vivo cloning
  • MAP Kinase
  • Random mutagenesis
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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