Hydroxyurea-induced global transcriptional suppression in mouse ES cells

Peng Cui, Qiang Lin, Chengqi Xin, Lu Han, Lili An, Yulan Wang, Zhishang Hu, Feng Ding, Lingfang Zhang, Songnian Hu*, Haiying Hang, Jun Yu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Hydroxyurea (HU), as a therapeutic medicine, has been extensively used clinically. To further survey molecular mechanisms of HU treatment, we analyzed global transcriptomic alteration of mouse ES cells in response to the treatment using high-throughput sequencing. We show that the global transcriptional activity is significantly suppressed as cells are exposed to HU treatment and alters multiple key cellular pathways, including cell cycle, apoptosis and DNAs. HU treatment also alters alternative splicing mechanisms and suppresses non-coding RNA expression. Our result provides novel clues for the understanding of how cells respond to HU and further suggests that high-throughput sequencing technology provides a powerful tool to study mechanisms of clinical drugs at the cellular level. Published by Oxford University Press 2010.

Original languageEnglish (US)
Pages (from-to)1661-1668
Number of pages8
JournalCarcinogenesis
Volume31
Issue number9
DOIs
StatePublished - May 31 2010

ASJC Scopus subject areas

  • Cancer Research

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