Human cardiac progenitor cell grafts as unrestricted source of supernumerary cardiac cells in healthy murine hearts

Giancarlo Forte, Stefano Pietronave, Giorgia Nardone, Andrea Zamperone, Eugenio Magnani, Stefania Pagliari, Francesca Pagliari, Cristina Giacinti, Carmine Nicoletti, Antonio Musaró, Mauro Rinaldi, Marco Ribezzo, Chiara Comoglio, Enrico Traversa, Teruo Okano, Marilena Minieri, Maria Prat, Paolo Di Nardo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.

Original languageEnglish (US)
Pages (from-to)2051-2061
Number of pages11
JournalStem Cells
Volume29
Issue number12
DOIs
StatePublished - Dec 1 2011

Keywords

  • Cardiac progenitor cells
  • Cardiac tissue engineering
  • Cell sheet technology

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Human cardiac progenitor cell grafts as unrestricted source of supernumerary cardiac cells in healthy murine hearts'. Together they form a unique fingerprint.

Cite this