High throughput second harmonic imaging for label-free biological applications

Carlos Macias Romero, Marie E P Didier, Pascal Jourdain, Pierre Marquet, Pierre J. Magistretti, Orly B. Tarun, Vitalijs Zubkovs, Aleksandra Radenovic, Sylvie Roke

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.
Original languageEnglish (US)
Pages (from-to)31102
JournalOptics Express
Volume22
Issue number25
DOIs
StatePublished - Dec 8 2014

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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