High Throughput Screening of Signal Transduction Mutants with Luciferase Imaging

Liming Xiong, Lisa David, Becky Stevenson, Jian Kang Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Arabidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.

Original languageEnglish (US)
Pages (from-to)159-170
Number of pages12
JournalPlant Molecular Biology Reporter
Volume17
Issue number2
StatePublished - 1999
Externally publishedYes

Keywords

  • CCD camera
  • Imaging
  • Luciferase
  • Mutant screening
  • Signal transduction

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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