High Sensitivity, High Selectivity SERS Detection of MnSOD Using Optical Nanoantennas Functionalized with Aptamers

Maximilien Cottat, Cristiano D'andrea, Ryohei Yasukuni, Natalia Malashikhina, Ruta Grinyte, Nathalie Lidgi-Guigui, Barbara Fazio, Angela Sutton, Olivier Oudar, Nathalie Charnaux, Valery Pavlov, Andrea Toma, Enzo Di Fabrizio, Pietro G. Gucciardi*, Marc Lamy De La Chapelle

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

In this paper, we present the development of a highly sensitive, specific and reproducible nanobiosensor to detect one specific liver cancer biomarker, the manganese super oxide dismutase (MnSOD). The high sensitivity and reproducibility was reached by using SERS on gold nanostructures (nanocylinders and coupled nanorods) produced by electron-beam lithography (EBL). The specificity of the detection was provided by the use of a specific aptamer with high affinity to the targeted protein as a recognition element. With such a sensor, we have been able to observe the SERS signal of the MnSOD at concentrations down to the nM level and to show with negative control that this detection is specific due to the use of the aptamer. This latter issue has allowed us to detect the MnSOD in different body fluids (serum and saliva) at concentrations in the nM range. We have then demonstrated the effectiveness of our SERS nanobiosensor using aptamer as a bioreceptor for the detection of disease biomarker at low concentration and in complex fluids.

Original languageEnglish (US)
Pages (from-to)15532-15540
Number of pages9
JournalJournal of Physical Chemistry C
Volume119
Issue number27
DOIs
StatePublished - Jul 9 2015

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Energy(all)
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films

Fingerprint

Dive into the research topics of 'High Sensitivity, High Selectivity SERS Detection of MnSOD Using Optical Nanoantennas Functionalized with Aptamers'. Together they form a unique fingerprint.

Cite this