High-performance protein separation by ion exchange membrane partitioned free-flow isoelectric focusing system

Jiuhua Cheng, Yi Li, Tai-Shung Chung*, Shing Bor Chen, William B. Krantz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The objective of this study is to investigate a combination of ion exchange membranes and free-flow isoelectric focusing (FFIEF) technology for high-selectivity and high-flux protein separation, in which ion exchange membranes are used as the separation media. An FFIEF device has been designed and extensive experiments have been conducted to prove its effectiveness in enhancing the protein separation performance. Three types of membranes were employed in this work and they were commercial microfiltration (MF) ion exchange membranes, commercial neutral UF cellulose membranes, and home-made ultrafiltration sulfonated polysulfone (UF SPSf) ion exchange membranes. The protein separation results show that the home-made UF SPSf membranes have the superior selectivity and flux to other membranes. This is due to the fact that a stable pH gradient across the membranes as well as the interaction between the protein molecules and membrane surface plays an important role in the high-performance protein separation. By applying a semi-batch separation process and optimizing various experimental conditions, a high-purity (> 90 %) and concentrated target protein is obtained at the permeation side of the home-made UF SPSf membranes with a high flux.

Original languageEnglish (US)
Pages (from-to)2241-2251
Number of pages11
JournalChemical Engineering Science
Volume63
Issue number8
DOIs
StatePublished - Apr 1 2008

Keywords

  • Electrophoresis
  • IEM-FFIEF (ion exchange membrane partitioned free-flow isoelectric focusing)
  • Membrane
  • Polymers
  • Protein mixture
  • Separation

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Applied Mathematics

Fingerprint Dive into the research topics of 'High-performance protein separation by ion exchange membrane partitioned free-flow isoelectric focusing system'. Together they form a unique fingerprint.

Cite this