High-affinity sulfonated materials with transition metal counterions for enhanced protein separation in dual-layer hollow fiber membrane chromatography

Yi Li, Tai-Shung Chung*, Sui Yung Chan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

High-affinity membrane materials have been successfully synthesized through a combination of the polymer sulfonation reaction with transition metal counterion exchange treatment. This type of promising materials were embodied for the first time with the aid of dual-layer hollow fiber technology for protein separation. Three types of immobilized metal affinity membranes (Cu2+, Ni2+ and Zn2+ forms) were developed in this work and they all exhibited enhanced protein separation performance compared to the as-spun hollow fiber in H+ form due to the strong affinity between transition metal counterions and target protein molecules. Ultimately, the high-purity target protein (>99%, w/w) could be achieved via the membrane in Cu2+ form.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalJournal of Chromatography A
Volume1187
Issue number1-2
DOIs
StatePublished - Apr 11 2008

Keywords

  • Affinity
  • Coordination
  • Dual-layer hollow fiber
  • Protein separation
  • Transition metal ions

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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